Simulation methods for liquid–liquid phase separation of disordered proteins
نویسندگان
چکیده
منابع مشابه
Methods for Separation of Proteins.
Most of the viethods available for general separation of proteins have been applied to the proteins of the crystalline lens. The classical work of Mb'rner, in 1894, suggested that there were three soluble lens proteins tohich he labeled a-, j6and y-crystallin and an insoluble residue which he called albuminoid. Subsequent attempts at fructionation using differential solubility, low-voltage elec...
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Liquid-liquid phase separation of intrinsically disordered proteins (IDPs) is a major undergirding factor in the regulated formation of membraneless organelles in the cell. The phase behavior of an IDP is sensitive to its amino acid sequence. Here we apply a recent random-phase-approximation polymer theory to investigate how the tendency for multiple chains of a protein to phase-separate, as ch...
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Phase transitions of linear multivalent proteins control the reversible formation of many intracellular membraneless bodies. Specific non-covalent crosslinks involving domains/motifs lead to system-spanning networks referred to as gels. Gelation transitions can occur with or without phase separation. In gelation driven by phase separation multivalent proteins and their ligands condense into den...
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ژورنال
عنوان ژورنال: Current Opinion in Chemical Engineering
سال: 2019
ISSN: 2211-3398
DOI: 10.1016/j.coche.2019.03.004